CLINICAL GRADE CELL LINES

FEEDER DEPENDENT (FD)

CELL LINE # HAD-C 100 PCB (MCB) HAD-C 100 2 CB (WCB) HAD-C 102 PCB (MCB) HAD-C 106 PCB (MCB)
TYPE hESCs hESCs hESCs hESCs
SEX Male Male Male Male
hPSCReg ID # HADe007-A HADe007-C HADe008-A HADe009-A
NIH APPROVAL # NIHhESC-11-0123 NIHhESC-11-0123 NIHhESC-11-0124 NIHhESC-11-0125
ASSOCIATED PUBLICATION Tannenbaum SE et al., Derivation of Xeno-Free and GMP-Grade Human Embryonic Stem Cells – Platforms for Future Clinical Applications. June 20, 2012. PLoS One
RELATED PRODUCTS ●HAD-C 100 Matched Research-grade Bank (RG); ●HAD-C 100 Feeder-Free (FF) Seed Cell Bank;●HAD-C 100 WCB Feeder-Dependent (FD) Cell Line; ●HAD-C 100 PCB Feeder-Dependent (FD) Cell Line; ●HAD-C 102 Matched Research-grade Bank; ●HAD-C 102 Feeder-Free (FF) Seed Cell Bank; ●HAD-C 106 Matched Research-grade Bank (RG); ●HAD-C 106 Feeder-Free (FF) Seed Cell Bank;
CULTURE PLATFORM CellGro; Umbilical Cord Feeders CellGro; Umbilical Cord Feeders CellGro; Umbilical Cord Feeders CellGro; Umbilical Cord Feeders
PASSAGE # P11 P16 P20 P24
PASSAGING METHOD Manual Passaging Manual Passaging Manual Passaging Manual Passaging
CELLS FROZEN AS Cell Clusters Cell Clusters Cell Clusters Cell Clusters
DOCUMENTATION AVAILABLE Embryo Donor Testing Results
QC Characterization Results
Safety Testing Results (Hy Laboratories, Rehovot--Israel; Bioreliance--UK if available)
Quality System Statements for GMP Facility (Hadassah, Israel)
GMP Certificate for Hadassah GMP Facility (Ministry of Health, Israel)
List of Hadassah hESC Research Center QA SOPs for hESC Derivation, Culture and Banking
List of Raw Materials (catalogue and lot numbers) for the Generation, Maintenance and Banking
Brief Summary of the Donor Medical History
Information About the Embryo (gamete donation) for the relevant HAD line
Information About the Cytogenetic Stability of line
Certificate of Analysis for the Relevant HAD line
Ethics Committee Documents from IRB
Additional Documentation Available as Necessary
Images Picture1 HAD-C 100 1O CB
β-TUBULIN 		Picture1 HAD-C 100 2O CB
SSEA3 		Untitled HAD-C 102 1O CB
m-actin/DAPI 		Untitled HAD-C 106 1O CB
SOX-17/DAPI